Wallabies coach Michael Cheika had named an unchanged team for Saturday's big clash with England at Twickenham, however lock Adam Coleman failed a fitness test on his injured thumb.
The lock failed a final fitness test on Friday morning, with 26-year old Blake Enever straight into the starting side and set to debut against England on Saturday.
The Brumbies lock will become Wallaby 916 when he runs out at Twickenham, and the Wallabies 26th debutant since the Rugby World Cup Final in 2015 at the same venue.
Michael Cheika and his coaching staff have also finalised the reserves with the Wallabies opting for a six-two split on the bench.
Lopeti Timani is the sole inclusion with Henry Speight dropping off the extended bench that was named yesterday.
Fresh off a 29-21 win over Wales in Cardiff, the Wallabies will be aiming to turn the tide on England.
The Wallabies haven't tasted victory over the old enemy since the 2015 Rugby World Cup and have since lost their last four games to England.
Stephen Moore will become the seventh most-capped player of all-time when he runs out for his 128th game in the Wallaby jersey on Saturday, in what will be his penultimate Test.
Wallabies coach Michael Cheika said Coleman wasn't completely fit, despite training better than expected during the week.
"He is not 100 per cent. I would be lying if said he was 100 and you know I never lie," he said.
Michael Cheika has also backed Samu Kerevi in against England, keeping the Reds centre at 12, with Karmichael Hunt on the bench for the second week in a row.
Kerevi has made the most of his starting opportunities in the last fortnight, showing his progress in defence.
Though he wasn't as dominant against Wales as he was against Japan, Kerevi did enough to hold out Hunt in that starting lineup.
Cheika said Kerevi and Hunt had worked as a tag team well against Wales.
"Samu had more of a challenge last week, but he fought through it. Then together, they did the job quite nicely between the two of them," he said.
"Karmichael has been injured a fair bit before we got into this tour, maybe he'll get more minutes this week.
"I am a big fan of Samu. The more time he gets the better he is going to be."
1. Scott Sio (41 Tests)
2. Tatafu Polota-Nau (80 Tests)
3. Sekope Kepu (89 Tests)
4. Rob Simmons (80 Tests)
5. Blake Enever*
6. Ned Hanigan (11 Tests)
7. Michael Hooper (c) (77 Tests)
8. Sean McMahon (24 Tests)
9. Will Genia (vc) (86 Tests)
10. Bernard Foley (vc) (53 Tests)
11. Reece Hodge (22 Tests)
12. Samu Kerevi (16 Tests)
13. Tevita Kuridrani (56 Tests)
14. Marika Koroibete (6 Tests)
15. Kurtley Beale (69 Tests)
16. Stephen Moore (127 Tests)
17. Tom Robertson (17 Tests)
18. Allan Alaalatoa (21 Tests)
19. Matt Philip (2 Tests)
20. Ben McCalman (51 Tests)
21. Lopeti Timani (10 Tests)
22. Nick Phipps (60 Tests)
23. Karmichael Hunt (4 Tests)